生物
内生真菌在植物防御中的应用
抗菌剂
琼脂
致病菌
琼脂扩散试验
微生物学
细菌
琼脂平板
分离(微生物学)
植物
金黄色葡萄球菌
遗传学
作者
Zi-Ning Liang,Hua Zhu,Kaiping Lai,Long Chen
出处
期刊:PubMed
日期:2014-04-01
卷期号:37 (4): 564-8
被引量:4
摘要
To isolate and identify endophytic fungi from Brucea javanica, and to detect the antimicrobial activity of these strains.Endophytic fungi were isolated by tissue inoculation culture and identified by conventional morphological characteristic method. Seven kinds of pathogenic fungi and three kinds of bacteria were used as targeting microbes to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods, respectively.A total of 83 endophytic fungi strains were isolated from the root, stem, leaf and fruit of Brucea javanica. 34 strains were obtained from the stem, 32 strains were obtained from the leaf, 15 strains were isolated from the root and 2 strains came from the fruit. These 73 strains which had been identified attribute to 5 orders, 6 families and 12 genera. For the isolated strains, 14 strains had antifungal activities against at least one pathogenic fungi, 9 strains showed antibacterial activities against one or more bacteria. Especially, the strain YJ-17 which belonged to Phomopsis genus showed the best inhibitory effect on the targeting microbes.The endophytic fungi from Brucea javanica show diversity and microbial inhibition activity, and are worthy for further study on plant disease controlling.
科研通智能强力驱动
Strongly Powered by AbleSci AI