荧光团
免疫荧光
直接荧光抗体
抗体
化学
计算生物学
生物
免疫学
荧光
光学
物理
作者
Ian D. Odell,Deborah Cook
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 111-117
被引量:6
标识
DOI:10.1007/978-1-4939-1050-2_6
摘要
Immunofluorescence is a laboratory technique that utilizes a fluorophore-labeled antibody to detect immune complexes in tissue. Most of the labeled antibodies used in a clinical laboratory bind the conserved domains within each class of human antibodies, allowing them to detect a wide range of autoimmune complexes. Drawbacks to this technique mostly relate to proper handling of the specimen and the fluorophore-labeled antibodies. Therefore, having a basic understanding of fluorophores and antibodies is important for processing a specimen that yields a high signal-to-background ratio as well as troubleshooting problems, should they arise.
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