假基因
基因
生物
遗传学
内含子
克隆(编程)
基因家族
序列分析
外显子
基因组
计算机科学
程序设计语言
摘要
Caffeine is an important bioactive compound in tea.It is synthesized from xanthosine catalysed by N-methyltransferase(NMT) that using S-adenosylmethionine(SAM) as the methyl donor.Based on the characterization of high similarity of CS(Caffeine Synthase) gene sequences,6 NMTs were cloned from Baiye 1 by using the technique of long PCR and cloning flanking DNA sequence.Two of them are TCS1 and TCS2,one is a pseudogene,and the other three are named TCS3,TCS4 and TCS5,respectively.It was found that these genes were all composed by four exons and three introns through analysis of gene structure.The NMTs of Camellia plants were clustered into 5 groups,the similarity of TCS4 and TCS5 between with other genes is lower.These results provided useful references to dissect the genetic mechanism of caffeine from genome lev el in tea plant for the future.
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