微生物群
DNA测序
计算生物学
生物
工作流程
基因组
生物信息学
扩增子测序
哼
管道(软件)
16S核糖体RNA
遗传学
DNA
基因
计算机科学
数据库
艺术史
艺术
程序设计语言
表演艺术
作者
Ranjit Kumar,Peter Eipers,Rebecca B. Little,Michael R. Crowley,David K. Crossman,Elliot J. Lefkowitz,Casey D. Morrow
标识
DOI:10.1002/0471142905.hg1808s82
摘要
Abstract Historically, in order to study microbes, it was necessary to grow them in the laboratory. It was clear though that many microbe communities were refractory to study because none of the members could be grown outside of their native habitat. The development of culture‐independent methods to study microbiota using high‐throughput sequencing of the 16S ribosomal RNA gene variable regions present in all prokaryotic organisms has provided new opportunities to investigate complex microbial communities. In this unit, the process for a microbiome analysis is described. Many of the components required for this process may already exist. A pipeline is described for acquisition of samples from different sites on the human body, isolation of microbial DNA, and DNA sequencing using the Illumina MiSeq sequencing platform. Finally, a new analytical workflow for basic bioinformatics data analysis, QWRAP, is described, which can be used by clinical and basic science investigators. Curr. Protoc. Hum. Genet . 82:18.8.1‐18.8.29. © 2014 by John Wiley & Sons, Inc.
科研通智能强力驱动
Strongly Powered by AbleSci AI