The protective effects of lncRNA ZFAS1/miR-421-3p/MEF2C axis on cerebral ischemia-reperfusion injury

生物 MEF2C公司 细胞凋亡 小RNA 细胞生物学 下调和上调 分子生物学 基因表达 生物化学 基因
作者
Jiangqi Xu,Xiaohong Huang,Shixiang Liu,Dongdong Chen,Yufang Xie,Zhenwu Zhao
出处
期刊:Cell Cycle [Informa]
卷期号:21 (18): 1915-1931 被引量:3
标识
DOI:10.1080/15384101.2022.2060627
摘要

LncRNA ZNFX1 antisense RNA 1 (ZFAS1) could improve neuronal damage and inhibit inflammation and apoptosis. We conducted an in-depth exploration on the protective mechanism of ZFAS1 in cerebral ischemia-reperfusion injury. Overexpressed or silenced plasmids of ZFAS1 were transfected into the cells to analyze the effects of oxygen-glucose deprivation/reperfusion (OGD/R) treatment on the viability, apoptosis and related gene expressions of Neuro-2a cell by performing MTT assay, flow cytometry, qRT-PCR, and Western blot. Bioinformatic analysis, qRT-PCR, dual-luciferase reporter assay and RNA immunoprecipitation were used to screen and verify the miRNA(s) which could competitively bind with ZFAS1 and downstream mRNA(s) targeted by the miRNA(s). The effects of ZFAS1 and the above target miRNA(s) or gene(s) on the apoptosis of OGD/R-injured cells, apoptosis-related proteins, inflammatory factors and p65/IκBα pathway were further verified via the rescue test. The results from the middle cerebral artery occlusion (MCAO) mouse model in vivo were consistent with those from the cellular experiments. The expression of lncRNA ZFAS1 in OGD/R-injured cells was inhibited, and the up-regulation of ZFAS1 protected Neuro-2a cells. MiR-421-3p was predicted to be the target miRNA of ZFAS1 and could offset the protective effect of ZFAS1 overexpression on OGD/R-injured cells following its up-regulation. MEF2C, which was the downstream target gene of miR-421-3p, reversed the OGD/R-induced enhanced cell damage caused by miR-421-3p mimic when MEF2C was overexpressed. In in vivo studies, ZFAS1 overexpression reduced brain tissue infarction, apoptosis and gene regulation caused by MCAO, while miR-421-3p mimic had the opposite effect. Collectively, the regulation of lncRNA ZFAS1/miR-421-3p/MEF2C axis showed protective effects on cerebral ischemia-reperfusion injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
shylockcai发布了新的文献求助10
1秒前
lyl完成签到,获得积分10
1秒前
Jocd完成签到,获得积分10
1秒前
Akim应助孙二二采纳,获得10
2秒前
优雅的千凝完成签到,获得积分10
2秒前
Time完成签到,获得积分10
3秒前
格拉希尔完成签到 ,获得积分10
4秒前
Yzh完成签到,获得积分10
5秒前
shuke完成签到,获得积分10
6秒前
啊七飞完成签到,获得积分10
6秒前
琦琦国王完成签到,获得积分10
7秒前
务实的绝悟完成签到,获得积分10
10秒前
经纲完成签到 ,获得积分0
10秒前
我说苏卡你说不列完成签到,获得积分10
12秒前
CipherSage应助科研通管家采纳,获得10
14秒前
14秒前
顾矜应助科研通管家采纳,获得10
14秒前
14秒前
劲秉应助科研通管家采纳,获得10
14秒前
sun应助科研通管家采纳,获得20
14秒前
苗条绝义应助科研通管家采纳,获得10
15秒前
隐形曼青应助科研通管家采纳,获得10
15秒前
科研通AI5应助科研通管家采纳,获得10
15秒前
蔡从安完成签到,获得积分20
15秒前
劲秉应助科研通管家采纳,获得10
15秒前
CipherSage应助科研通管家采纳,获得10
15秒前
NexusExplorer应助科研通管家采纳,获得10
15秒前
黑囡应助科研通管家采纳,获得10
15秒前
15秒前
科研通AI5应助科研通管家采纳,获得10
15秒前
科研通AI2S应助科研通管家采纳,获得10
15秒前
5165asd完成签到 ,获得积分10
16秒前
ritata完成签到 ,获得积分10
17秒前
小彤完成签到 ,获得积分10
18秒前
18秒前
ll应助蔡从安采纳,获得10
19秒前
boltos完成签到,获得积分20
20秒前
善学以致用应助橙猫猫采纳,获得10
21秒前
GEZIKU完成签到 ,获得积分10
23秒前
24秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 720
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3566811
求助须知:如何正确求助?哪些是违规求助? 3139560
关于积分的说明 9431989
捐赠科研通 2840353
什么是DOI,文献DOI怎么找? 1560990
邀请新用户注册赠送积分活动 730141
科研通“疑难数据库(出版商)”最低求助积分说明 717855