Construction and applications of the EOMA spheroid model of Kaposiform hemangioendothelioma

球体 川地31 癌症研究 下调和上调 细胞生物学 生物 体外 病理 医学 血管生成 基因 遗传学
作者
Yanan Li,Xinglong Zhu,Li Li,Cui Bao,Qin Liu,Ning Zhang,Ziyan He,Yi Ji,Ji Bao
出处
期刊:Journal of Biological Engineering [Springer Nature]
卷期号:18 (1)
标识
DOI:10.1186/s13036-024-00417-4
摘要

Kaposiform hemangioendothelioma (KHE) is a rare intermediate vascular tumor with unclear pathogenesis. Recently, three dimensional (3D) cell spheroids and organoids have played an indispensable role in the study of many diseases, such as infantile hemangioma and non-involuting congenital hemangiomas. However, few research on KHE are based on the 3D model. This study aims to evaluate the 3D superiority, the similarity with KHE and the ability of drug evaluation of EOMA spheroids as an in vitro 3D KHE model.After two days, relatively uniform morphology and high viability of EOMA spheroids were generated by the rotating cell culture system (RCCS). Through transcriptome analysis, compared with 2D EOMA cells, focal adhesion-related genes such as Itgb4, Flt1, VEGFC, TNXB, LAMA3, VWF, and VEGFD were upregulated in EOMA spheroids. Meanwhile, the EOMA spheroids injected into the subcutaneous showed more obvious KMP than 2D EOMA cells. Furthermore, EOMA spheroids possessed the similar characteristics to the KHE tissues and subcutaneous tumors, such as diagnostic markers (CD31 and LYVE-1), cell proliferation (Ki67), hypoxia (HIF-1α) and cell adhesion (E-cadherin and N-cadherin). Based on the EOMA spheroid model, we discovered that sirolimus, the first-line drug for treating KHE, could inhibit EOMA cell proliferation and downregulate the VEGFC expression. Through the extra addition of VEGFC, the effect of sirolimus on EOMA spheroid could be weakened.With a high degree of similarity of the KHE, 3D EOMA spheroids generated by the RCCS can be used as a in vitro model for basic researches of KHE, generating subcutaneous tumors and drug screening.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小二完成签到,获得积分10
刚刚
刚刚
橙汁橙完成签到,获得积分10
1秒前
鱼丸发布了新的文献求助30
2秒前
咕噜噜完成签到,获得积分10
2秒前
怡然聪展发布了新的文献求助10
2秒前
鱼咬羊发布了新的文献求助10
3秒前
3秒前
xuan完成签到,获得积分10
4秒前
泓沐关注了科研通微信公众号
4秒前
4秒前
4秒前
4秒前
88完成签到 ,获得积分10
5秒前
5秒前
小泥娃完成签到 ,获得积分10
5秒前
Linyi发布了新的文献求助10
5秒前
6秒前
领导范儿应助lina采纳,获得10
6秒前
7秒前
尘野发布了新的文献求助10
7秒前
7秒前
西西弗斯发布了新的文献求助10
8秒前
科研通AI5应助wyh99采纳,获得10
8秒前
王加通完成签到,获得积分10
9秒前
郭小胖14发布了新的文献求助10
9秒前
9秒前
魔m完成签到,获得积分10
9秒前
Hover发布了新的文献求助10
10秒前
11秒前
Orange应助单纯孤兰采纳,获得100
11秒前
xioatudou完成签到,获得积分10
11秒前
222发布了新的文献求助10
12秒前
合适冷霜应助喜金少女采纳,获得10
12秒前
虚幻的翎应助bal采纳,获得30
13秒前
666发布了新的文献求助20
13秒前
MFiWanting发布了新的文献求助10
13秒前
Pumpkin应助hyper3than采纳,获得10
14秒前
YULIA完成签到,获得积分10
14秒前
14秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546815
求助须知:如何正确求助?哪些是违规求助? 3123829
关于积分的说明 9357111
捐赠科研通 2822447
什么是DOI,文献DOI怎么找? 1551477
邀请新用户注册赠送积分活动 723475
科研通“疑难数据库(出版商)”最低求助积分说明 713766