Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

普鲁士蓝 过氧化氢 介孔材料 材料科学 纳米技术 催化作用 无机化学 核化学 化学 有机化学 电化学 电极 物理化学 冶金
作者
Rajesh Madhuvilakku,Yonggeun Hong,Irin Sultana Nila,Vanina Myuriel Villagra Moran,Subramanian Palanisamy,Zeeshan Ahmad Khan,Sehoon Jeong,Sang Guan You
出处
期刊:ACS Applied Materials & Interfaces [American Chemical Society]
卷期号:15 (48): 55466-55485 被引量:7
标识
DOI:10.1021/acsami.3c13594
摘要

Despite the effectiveness and selectivity of natural enzymes, their instability has paved the way for developing nanozymes with high peroxidase activity using a straightforward technique, thereby expanding their potential for multifunctional applications. Herein, meso-copper-Prussian blue microcubes (Meso-Cu-PBMCs) nanozymes were successfully prepared via a cost-effective hydrothermal route. It was found that the Cu-PBMCs nanozymes, with three-dimensional (3D) mesoporous cubic morphologies, exhibited an excellent peroxidase-like property. Based on the high affinity of Meso-Cu-PBMCs toward H2O2 (Km = 0.226 μM) and TMB (Km = 0.407 mM), a colorimetric sensor for in situ H2O2 detection was constructed. On account of the high catalytic activity, affinity, and cascade strategy, the Meso-Cu-PBMCs nanozyme generated rapid multicolor displays at varying H2O2 concentrations. Under optimized conditions, the proposed sensor exhibits a preferable sensitivity of 18.14 μA μM-1, a linear range of 10 nM-25 mM, and a detection limit of 6.36 nM (S/N = 10). The reliability of the sensor was verified by detecting H2O2 in spiked human blood serum and milk samples, as well as by detecting in situ H2O2 generated from the neuron cell SH-SY5Y. Besides, the Meso-Cu-PBMCs nanozyme facilitated the catalysis of H2O2 in cancer cells, generating •OH radicals that induce the death of cancer cells (HCT-116 colon cancer cells), which holds substantial potential for application in chemodynamic therapy (CDT). This proposed strategy holds promise for simple, rapid, inexpensive, and effective intracellular biosensing and offers a novel approach to improve CDT efficacy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
1秒前
2秒前
立仔发布了新的文献求助10
3秒前
ShengzhangLiu发布了新的文献求助10
3秒前
3秒前
SYLH应助科学采纳,获得10
3秒前
4秒前
Salt发布了新的文献求助10
5秒前
JamesPei应助科研眼镜蛇采纳,获得10
6秒前
听安完成签到,获得积分10
7秒前
77关闭了77文献求助
7秒前
充电宝应助lzr采纳,获得10
8秒前
苗苗发布了新的文献求助10
8秒前
称心凡霜完成签到,获得积分10
13秒前
14秒前
一颗石榴完成签到,获得积分10
14秒前
温离完成签到,获得积分10
15秒前
ZZ发布了新的文献求助10
18秒前
玉昆完成签到 ,获得积分10
19秒前
今后应助吴玉婷采纳,获得30
19秒前
立仔完成签到,获得积分10
20秒前
Zoe_Zhang完成签到 ,获得积分10
21秒前
22秒前
重要的秋尽完成签到,获得积分10
23秒前
111发布了新的文献求助10
23秒前
量子星尘发布了新的文献求助10
23秒前
lzr完成签到,获得积分10
26秒前
26秒前
27秒前
包佳梁完成签到,获得积分10
29秒前
如意含雁关注了科研通微信公众号
29秒前
寒江孤影完成签到,获得积分10
31秒前
ddd123发布了新的文献求助10
31秒前
33秒前
苗苗完成签到,获得积分10
38秒前
Atec发布了新的文献求助10
38秒前
李健的小迷弟应助无名采纳,获得10
39秒前
40秒前
40秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975458
求助须知:如何正确求助?哪些是违规求助? 3519866
关于积分的说明 11199996
捐赠科研通 3256213
什么是DOI,文献DOI怎么找? 1798133
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305