Ubiquitylome analysis reveals the involvement of ubiquitination in the cold responses of banana seedling leaves

苗木 蛋白质组 生物 非生物胁迫 泛素 细胞生物学 转录因子 植物 园艺 生物化学 基因
作者
Wei Lin,Shuijin Wu,Wei Mi
出处
期刊:Journal of Proteomics [Elsevier BV]
卷期号:288: 104994-104994 被引量:1
标识
DOI:10.1016/j.jprot.2023.104994
摘要

Low temperature is a crucial environmental factor limiting the productivity and distribution of banana. Ubiquitination (Kub) is one of the main posttranslational modifications (PTMs) involved in plant responses to abiotic stresses. However, little information is available on the effects of Kub on banana under cold stress. In this study, we used label-free quantification (LFQ) to identify changes in the protein expression and Kub levels in banana seedling leaves after chilling treatment. In total, 4156 proteins, 1089 ubiquitinated proteins and 2636 Kub sites were quantified. Western blot assays showed that Kub was abundant in leaves after low-temperature treatment. Our results show that the proteome and ubiquitylome were negatively correlated, indicating that Kub could be involved in the degradation of proteins in banana after chilling treatment. Based on bioinformatics analysis, low-temperature stress-related signals and metabolic pathways such as cold acclimation, glutathione metabolism, calcium signaling, and photosynthesis signaling were identified. In addition, we found that transcription factors and chromatin remodeling factors related to low-temperature stress were ubiquitinated. Overall, our work presents the first systematic analysis of the Kub proteome in banana under cold stress and provides support for future studies on the regulatory mechanisms of Kub during the cold stress response in plants. Banana is a typical tropical fruit tree with poor low-temperature tolerance,however, the role of PTMs such as Kub in the cold response of banana remains unclear. This study highlights the fact that the effects of low-temperature on proteome and ubiquitylome in the banana seedling leaves, we discussed the correlation between transcriptome and proteome, ubiquitylome and proteome, and we analyzed the expression and the changes of ubiquitination levels of low-temperature related proteins and pathway after chilling treatment, and we found that transcription factors and chromatin remodeling factors related to low-temperature stress were ubiquitinated. This study provides new insights into the ubiquitination pathway of banana under cold stress.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
暮夕梧桐完成签到,获得积分10
1秒前
嘻嘻哈哈完成签到 ,获得积分10
5秒前
新洸完成签到 ,获得积分10
6秒前
卡卡西的猫完成签到 ,获得积分10
17秒前
聂青枫完成签到,获得积分10
23秒前
LOST完成签到 ,获得积分10
24秒前
yinyin完成签到 ,获得积分10
24秒前
沉静香氛完成签到 ,获得积分10
30秒前
32秒前
北笙完成签到 ,获得积分10
34秒前
卢健辉发布了新的文献求助10
38秒前
mark33442完成签到,获得积分10
39秒前
明天过后完成签到,获得积分10
41秒前
zhangxin完成签到,获得积分10
42秒前
一行白鹭上青天完成签到 ,获得积分10
44秒前
卢健辉完成签到,获得积分10
44秒前
汉堡包应助迷路的煎蛋采纳,获得10
45秒前
lightman完成签到,获得积分10
49秒前
51秒前
52秒前
欧阳小枫完成签到 ,获得积分10
52秒前
重要铃铛完成签到 ,获得积分10
56秒前
56秒前
57秒前
Hello应助Ying采纳,获得20
57秒前
amy完成签到,获得积分10
1分钟前
ivyjianjie完成签到 ,获得积分10
1分钟前
DaDA完成签到 ,获得积分10
1分钟前
晚晚完成签到,获得积分10
1分钟前
大陆完成签到,获得积分0
1分钟前
猪猪女孩完成签到,获得积分10
1分钟前
欢喜板凳完成签到 ,获得积分10
1分钟前
lu完成签到,获得积分10
1分钟前
郭元强完成签到,获得积分10
1分钟前
小许完成签到 ,获得积分10
1分钟前
1分钟前
xiaofeiyan完成签到 ,获得积分10
1分钟前
llhh2024完成签到,获得积分10
1分钟前
心系天下完成签到 ,获得积分10
1分钟前
David完成签到 ,获得积分10
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968559
求助须知:如何正确求助?哪些是违规求助? 3513391
关于积分的说明 11167370
捐赠科研通 3248808
什么是DOI,文献DOI怎么找? 1794465
邀请新用户注册赠送积分活动 875116
科研通“疑难数据库(出版商)”最低求助积分说明 804664