微泡
化学
小RNA
检出限
癌症生物标志物
癌症研究
转移
原位
聚合酶链反应
癌症
分子生物学
计算生物学
色谱法
生物
生物化学
内科学
基因
医学
有机化学
作者
Wei‐xian Zhang,Li Du,Yong‐Liang Yu,Jianhua Wang,Shuai Chen,Yong‐Liang Yu
出处
期刊:Talanta
[Elsevier]
日期:2024-01-01
卷期号:266: 125145-125145
被引量:3
标识
DOI:10.1016/j.talanta.2023.125145
摘要
Exosomal miRNAs can reflect tumor progression and metastasis, and are effective biomarkers for cancer diagnosis. However, the accuracy of exosomal miRNA-based cancer diagnosis is limited by the low sensitivity and complicated RNA extraction of traditional approaches. Herein, a novel biosensor is developed for in situ, extraction-free, and highly sensitive analysis of exosomal miRNAs via nanoflare combined with catalyzed hairpin assembly (CHA) amplification. Without cumbersome and costly miRNA extraction or transfection agents, nanoflare can directly enter the exosomes to bind target miRNAs and generate a fluorescence signal that can be amplified by the CHA reaction to achieve the in situ and highly sensitive detection of exosomal miRNAs. Under the optimal conditions, the detection limit of 5 aM is obtained for three exosomal miRNAs, which is an order of magnitude lower than quantitative real time polymerase chain reaction (qRT-PCR). In combination with the linear discriminant analysis algorithm, five exosomes are distinguished with 100% accuracy. Importantly, five cancers including breast, lung, liver, cervical, and colon cancer from 64 patients are distinguished with 99% accuracy by testing exosomal miRNAs in clinical plasma. This simple, accurate, and sensitive biosensor holds the potential to be expanded into clinical non-invasive cancer diagnostic tests.
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