Absolute quantification of the microbiota spatial distribution in the murine large intestine

细菌 盲肠 肠道菌群 粪便 生物 微生物群 微生物学 大肠 管腔(解剖学) 免疫学 细胞生物学 生态学 遗传学 生物化学
作者
Wenyu Jin,Jiaxin Guo,Meng Zhang,Lin-Zuo Teng,Yanjie Chao,Philippe Joseph Sansonetti,Yi‐Zhou Gao
标识
DOI:10.59717/j.xinn-life.2023.100030
摘要

<p>The species diversity, abundance, and location of intestinal bacteria bear major consequences on host intestinal functions, affecting the balance between health and disease. Recent studies addressed the relative abundance of bacteria in feces and on the gut mucosal surface. However, few of these publications reported the absolute number of bacteria in different compartments of the gut, especially those associated with the intestinal epithelial surface, compared to the luminal content reflected by feces. In this study, the number of bacteria present in the lumen or associated with the mucosal surface of mice gut samples was calculated using the bacterial “internal standard method” during sequencing. Three spiking bacteria were used for mutual corroboration. The microbiota spatial distribution in the cecum, proximal and distal colon were studied in the lumen content and gut wall fixed niches. This study also provides an example to demonstrate that absolute and relative quantification may yield different conclusions. This study’s method may eliminate such misjudgments and provide insight into the distribution and quantity of gut microbiome.</p>

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
1秒前
2秒前
木米的里发布了新的文献求助30
2秒前
2秒前
3秒前
3秒前
吴1发布了新的文献求助10
4秒前
陌上花开发布了新的文献求助10
5秒前
5秒前
5秒前
顾化蛹完成签到,获得积分10
5秒前
wanci应助聪明的天亦采纳,获得10
5秒前
猫了个喵应助陆靖易采纳,获得10
5秒前
Christal发布了新的文献求助10
6秒前
NexusExplorer应助紫紫吃菠菜采纳,获得10
7秒前
wph发布了新的文献求助10
7秒前
8秒前
Ava应助swordlee采纳,获得10
8秒前
8秒前
顾化蛹发布了新的文献求助10
8秒前
飞云发布了新的文献求助80
9秒前
9秒前
10秒前
田様应助彳亍采纳,获得10
11秒前
吕吕发布了新的文献求助10
12秒前
嗷嗷嗷啊发布了新的文献求助10
13秒前
13秒前
14秒前
zzz发布了新的文献求助10
14秒前
王小明发布了新的文献求助10
15秒前
兴奋硬币发布了新的文献求助10
15秒前
wu发布了新的文献求助10
16秒前
小滕发布了新的文献求助10
17秒前
19秒前
NexusExplorer应助旅途之人采纳,获得10
20秒前
丘比特应助guard采纳,获得10
20秒前
21秒前
HIHI完成签到,获得积分10
22秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Structural Load Modelling and Combination for Performance and Safety Evaluation 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3555383
求助须知:如何正确求助?哪些是违规求助? 3131010
关于积分的说明 9389629
捐赠科研通 2830491
什么是DOI,文献DOI怎么找? 1556069
邀请新用户注册赠送积分活动 726432
科研通“疑难数据库(出版商)”最低求助积分说明 715738