Enhancement of growth, survival, immunity and disease resistance in Litopenaeus vannamei, by the probiotic, Lactobacillus plantarum Ep-M17

Green ecological prevention and control technology is a hot spot for aquatic disease research in recent years, and lactic acid bacteria is an important type of probiotic widely used in aquaculture. In this study, a strain of Lactobacillus plantarum Ep-M17 was isolated from the intestine of healthy grouper, which showed good antibacterial activity in vitro. To investigate the application prospects of Ep-M17 as a probiotic, we added it to the diet and fed Litopenaeus vannamei, and then detected its influence on the growth performance, survival rate, disease resistance, intestinal tissue structure, gene transcription, and the flora in the gut of shrimp. The results showed that feeding Ep-M17 increased the specific growth rate, reduced the feed conversion rate, improved the survival rate, and achieved a 76.9% relative protection rate after Vibrio parahaemolyticus E1 infection in shrimp. Histological examination displayed that Ep-M17-fed shrimp had a thick intestinal villi layer, which enhanced the protection against pathogen damage. It was also found that Ep-M17 significantly increased the activity levels of immune and digestion-related enzymes SOD, CAT, TRY, AKP, LIP, and AMS in the gut of shrimp, especially after V. parahaemolyticus E1 infection, these enzymes increased significantly higher than that of control. Transcriptome analysis revealed that Ep-M17 activated significantly differential expression of genes in immune, nutritional, metabolic, and Signal Transduction-related pathways in the gut of shrimp. In addition, Ep-M17 enriched the bacterial diversity of the shrimp gut, with a significant increase in many low-abundance bacterial species, a significant decrease in the number of pathogenic bacteria like Vibrio, and a significant increase in the number of beneficial bacteria. The above results evaluated that Ep-M17 as a potential probiotic can promote the growth and improve the disease resistance of shrimp by regulating the nutritional immune response and flora of the intestine.