On-DNA-1,2,3-Triazole Formation via Click Reaction

Copper-catalyzed azide-alkyne cycloaddition (CuAAC) provides a simple and convenient strategy to synthesize diverse 1,2,3-triazoles for drug discovery. Described herein is a protocol for the CuSO4-catalyzed cycloaddition between alkynes and DNA-appended azides.