The increased nitrogen (N) input with low utilization rate in artificial N management has led to massive reactive N (Nr) flows, putting the Earth in a high-risk state. It is essential to recover and recycle Nr during or after Nr removal from wastewater to reduce N input while simultaneously mitigate Nr pollution in addressing the N stress. However, mechanisms for efficient Nr recovery during or after Nr removal remain unclear. Here, the occurrence of N risk and progress in wastewater treatment in recent years as well as challenges of the current technologies for N recovery from wastewater were reviewed. Through analyzing N conversion fluxes in biogeochemical N-cycling networks, microbial N assimilation through photosynthetic and heterotrophic microorganisms was highlighted as promising alternative for synergistic N removal and recovery in wastewater treatment. In addition, the prospects and gaps of Nr recovery from wastewater through microbial assimilation are discussed.