伤口愈合
肌成纤维细胞
哈卡特
细胞生物学
收缩(语法)
成纤维细胞
体外
化学
细胞迁移
体内
药理学
医学
生物
免疫学
病理
生物化学
纤维化
内科学
遗传学
作者
Jinrui Yang,Xiaoqiang Liu,Wenping Wang,Ying Chen,Jie Liu,Ze Zhang,Chao Wu,Xupin Jiang,Yi Liang,Jiaping Zhang
标识
DOI:10.1016/j.bioelechem.2022.108247
摘要
Electric fields (EFs) are thought to play a decisive role in wound healing. However, most studies focused on the effects of EF on single species of cells in vitro. Here, we aimed to investigate the coordination function of EFs on wound healing. Using a bamamini pig whole-layer wound model, we further evaluated the potential of EFs as a treatment modality by applying continuous and stable EF to the wound, and we found that EF promoted wound contraction and re-epithelialization in vivo, which accelerated wound healing. In vitro, we found that EFs significantly promoted the collective migration of HaCaT cells, guided HSF cells rearrangement, and promoted collagen secretion and myofibroblast transformation, and the electrotaxis of HaCaT cells was significantly enhanced on the collagen substrate and F-actin polarization at the leading edge of the cells was more pronounced. Overall, we determined that EF promotes wound contraction by promoting myofibrillar transformation, while accelerating the formation of collagen substrates, and the substrates could provide a good basis for electric field-guided re-epithelialization. EF may promote wound healing in multiple dimensions interaction and coordinate the whole process of wound healing. These findings provide support for the continued development of EF for wound treatment applications.
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