Emerging trends in the methodology of environmental toxicology: 3D cell culture and its applications

Human diseases and health concerns caused by environmental pollutants are globally emerging. Therefore, rapid and efficient evaluation of the effects of environmental pollutants on human health is essential. Due to the significant differences between humans and animals and the lack of physiologically related environments, animal models and two-dimensional (2D) culture cannot accurately describe toxicological effects and predict actual in vivo responses. To make up for the limitations of traditional environmental toxicology screening, three-dimensional (3D) culture has been developed. The 3D culture could provide a good organizational structure comparable to the complex internal environment of humans and produce a more realistic response to environmental pollutants, which has been used in drug development, toxicity evaluation, personalized therapy and biological mechanism research. The goal of environmental toxicology is to provide clues and support for the risk assessment and management of environmental pollutants. With the development of 3D culture that can reproduce specific physiological aspects loaded with specific cells that reflect human biology, interactions between pollutants and target tissues and organs can be explored to assess the acute and chronic adverse health effects of exposure to various environmental toxins. The 3D culture with great potential shows broad prospects in toxicology research and is expected to bridge the gap between 2D culture and animal models eventually. In this sense, we strongly recommend that 3D culture be used to identify and understand environmental toxins, which will greatly facilitate the public's comprehensive understanding of environmental toxins.