五三肽重复
RNA剪接
生物
胚乳
RNA编辑
内含子
基因
线粒体
细胞生物学
线粒体DNA
核糖核酸
遗传学
作者
Hang Yang,Yunlong Wang,Yunlu Tian,Xuan Teng,Zehui Lv,Jie Lei,Erchao Duan,Hui Dong,Xue Yang,Yuanyan Zhang,Yinglun Sun,Xiaoli Chen,Xiuhao Bao,Rongbo Chen,Chuanwei Gu,Yipeng Zhang,Xiaokang Jiang,Wenyu Ma,Pengcheng Zhang,Yi Ji,Yu Zhang,Yihua Wang,Jianmin Wan
摘要
Most of the reported P-type pentatricopeptide repeat (PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22 (flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR protein. Mutation of FLO22 resulting in defective trans-splicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22 mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly up-regulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA.
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