化学
葡萄糖醛酸化
代谢途径
色谱法
体内
代谢组学
质谱法
党参
串联质谱法
根(腹足类)
微粒体
体外
谷胱甘肽
新陈代谢
生物化学
酶
生物技术
生物
医学
植物
替代医学
中医药
病理
作者
Qi Xie,Hanxue Wang,Huida Guan,Nan Xu,Xiang Zhao,Xuemei Cheng,Changhong Wang
标识
DOI:10.1016/j.jpba.2022.115140
摘要
Polyacetylenes, lobetyol, lobetyolin and lobetyolinin, are responsible for antitumor, antioxidant, anti-inflammatory, immunomodulatory activities of Codonopsis Radix. However, their metabolic pathways are still unknown. The study was purposed to investigate the metabolism of three polyacetylenes in vitro and in vivo by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Moreover, a rapid, sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous quantitative and semi-quantitative determination of lobetyol and its 12 metabolites to investigate the metabolic stability and metabolic phenotypes. A total of 47, 30 and 34 metabolites of lobetyol, lobetyolin and lobetyolinin were found in all samples. These metabolites are produced through extensive pathways, mainly involving oxidation, glucuronidation and glutathione conjugation. Lobetyol showed good metabolic stability in liver microsomes. The results of both recombinant human CYP enzymes and chemical inhibition experiments confirmed that CYP2C19, 1A1, 2C9, and 1A2 are the major isozymes mediating lobetyol metabolism.
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