光遗传学
多路复用
色阶
蛋白质工程
计算机科学
绿灯
合成生物学
计算生物学
定向进化
蓝光
纳米技术
生物
基因
神经科学
物理
光电子学
遗传学
材料科学
电信
光学
突变体
酶
生物化学
作者
Jaewan Jang,Kun Tang,Jeffrey Youn,Sherin McDonald,Hannes M. Beyer,Matías D. Zurbriggen,Maruti Uppalapati,G. Andrew Woolley
出处
期刊:Nature Methods
[Springer Nature]
日期:2023-02-23
卷期号:20 (3): 432-441
被引量:12
标识
DOI:10.1038/s41592-023-01764-8
摘要
Optogenetic tools for controlling protein-protein interactions (PPIs) have been developed from a small number of photosensory modules that respond to a limited selection of wavelengths. Cyanobacteriochrome (CBCR) GAF domain variants respond to an unmatched array of colors; however, their natural molecular mechanisms of action cannot easily be exploited for optogenetic control of PPIs. Here we developed bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s by engineering synthetic light-dependent interactors for a red/green GAF domain. The systematic approach enables the future engineering of the broad chromatic palette of CBCRs for optogenetics use. BICYCLs are among the smallest optogenetic tools for controlling PPIs and enable either green-ON/red-OFF (BICYCL-Red) or red-ON/green-OFF (BICYCL-Green) control with up to 800-fold state selectivity. The access to green wavelengths creates new opportunities for multiplexing with existing tools. We demonstrate the utility of BICYCLs for controlling protein subcellular localization and transcriptional processes in mammalian cells and for multiplexing with existing blue-light tools.
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