Fast three-dimensional DNA walker based on space double-confinement catalytic hairpin assembly for ultrasensitive homogeneous electrochemiluminescence detection of sonic hedgehog

电化学发光 检出限 生物传感器 DNA 计算机科学 纳米技术 材料科学 化学 色谱法 生物化学
作者
Zhujun Ai,Xinmin Li,Rui Chen,Daobin Han,Huijie Bai,Shaoying Yan,Pengcheng Gu,Haiping Wu,Shijia Ding,Junman Chen,Hua Tang
出处
期刊:Sensors and Actuators B-chemical [Elsevier BV]
卷期号:384: 133631-133631 被引量:2
标识
DOI:10.1016/j.snb.2023.133631
摘要

DNA walkers have been widely used to build biosensing systems. However, their moving efficiency was limited, which has hindered practical applications. Here, we for the first time integrated localized CHA with nanoscale AuNPs scaffolds to achieve space double-confined catalytic hairpin assembly (SDCHA) for developing a fast three-dimensional DNA walker. Driven by spatially proximal displacement, the walker possessed a high walking speed, which was about 8 times, 9 times and 23 times faster than LCHA, CHA-AuNPs and conventional CHA, respectively. Moreover, the walking time was about 1.4–8.3 times shorter than most recently reported DNA walkers and space-confined strand displacement reactions. By further integrating it with magnetic probes and g-C3N4 nanosheets, an ultrasensitive and enzyme-free homogeneous electrochemiluminescence (Ho-ECL) aptasensor was firstly constructed for sonic hedgehog (SHh) detection. Profiting from this tailored integration, the Ho-ECL aptasensor avoided multiple interferences from complex milieu as well as tedious procedures of electrode modifying and washing, showing good signal stability, repeatability and reliability. It was also proved to achieve ultrasensitive SHh detection with the detection limit as low as 13.5 fM, superior to previous research on SHh detection. This work might broaden ideas for the construction of fast DNA walkers and facile Ho-ECL bioanalytical methods for clinical diagnosis.
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