Three‐Dimensional Graphene Network Decorated with Bimetallic Cerium/Copper Oxide Nanoparticles for Non‐Enzymatic Diagnosis of Phenylketonuria

Abstract Exceptional sensitivity and user‐friendly operation of electrochemical sensors have made them a potential promise in diagnosing, monitoring, and treating Phenylketonuria (PKU). A three‐dimensional graphene matrix decorated with cerium and copper oxide particles was synthesized by a one‐pot hydrothermal technique. Because of several functional groups and active sites on the graphene composite, the fabricated sensor is able to detect PKU based on the non‐enzymatic sensing strategy. The bimetallic composite with excellent electrocatalyst activity accelerates the electrooxidation of phenylalanine. The as‐fabricated enzyme‐free sensor exhibited a wide linearity toward concentration of L‐phenylalanine (L‐Phe), from 50 to 8000 μ m with R 2 =0.9935 and a detection limit of 12 μ m (S/N=3). The experiments have been completed towards determining the L‐Phe concentrations in human serum with the recovery of 96 % and 105 %.