In Vitro Diagnosis and Visualization of Cerebral Ischemia/Reperfusion Injury in Rats and Protective Effects of Ferulic Acid by Raman Biospectroscopy and Machine Learning

阿魏酸 缺血 体外 药理学 医学 化学 神经科学 生物化学 麻醉 内科学 生物
作者
Mingying Liu,Ju Mu,Wan Gong,Kena Zhang,Maoyun Yuan,Yizhi Song,Bei Li,Naifu Jin,Wenjing Zhang,Dayi Zhang
出处
期刊:ACS Chemical Neuroscience [American Chemical Society]
卷期号:14 (1): 159-169 被引量:7
标识
DOI:10.1021/acschemneuro.2c00612
摘要

Ischemic stroke is a major cause of mortality with complicated pathophysiological mechanisms, and hematoxylin and eosin (HE) staining is a histochemical diagnosis technique heavily relying on subjective observation. In this study, we developed a noninvasive assay using Raman spectroscopy for in vitro diagnosis and visualization of cerebral ischemia/reperfusion injury and protective effects of ferulic acid. By establishing a middle cerebral artery occlusion (MCAO) model in Sprague-Dawley male rats, we found effective interventions by ferulic acid using the neurological function score and HE staining. Raman spectra of neuronal and neuroglial cells exhibited significant intensity changes of protein, nucleotide, lipid, and carbohydrate at 780, 814, 1002, 1012, 1176, 1224, 1402, 1520, 1586, 1614, and 1752 cm–1. Cluster vector analysis highlighted the alterations at 1002, 1080, 1298, 1430, 1478, 1508, 1586, and 1676 cm–1. To evaluate the levels of neuron injury and intervention performance, a random forest model was developed on Raman spectral data and achieved satisfactory accuracy (0.9846), sensitivity (0.9679–0.9932), and specificity (0.9945–0.9989), ranking peaks around 1002 cm–1 as key fingerprint for classification. Spectral phenylalanine-to-tryptophan ratio was the biomarker to visualize neuronal injury and intervention performance of ferulic acid with a resolution of 1 μm. Our results unravel the biochemical changes in neuronal cells with cerebral ischemia/reperfusion injury and ferulic acid treatment, and prove Raman spectroscopy coupled with machine learning as a power tool to classify neuron viability and evaluate the intervention performance in pharmacological research.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ZX0501完成签到,获得积分10
刚刚
所所应助爱吃粑粑采纳,获得10
1秒前
1秒前
1秒前
冷静的方盒完成签到,获得积分10
1秒前
小虎完成签到,获得积分10
2秒前
2秒前
微笑傥完成签到,获得积分10
2秒前
顾矜应助小申采纳,获得10
2秒前
伯赏秋白发布了新的文献求助10
3秒前
思源应助南敏株采纳,获得10
3秒前
马某完成签到,获得积分20
3秒前
阿曼尼完成签到 ,获得积分10
4秒前
小杨发布了新的文献求助10
4秒前
培乐多完成签到,获得积分10
4秒前
4秒前
我们完成签到,获得积分10
5秒前
摆烂的雨雨完成签到,获得积分10
6秒前
6秒前
7秒前
SciGPT应助Liu采纳,获得10
7秒前
大大怪发布了新的文献求助10
8秒前
yar应助感动城采纳,获得10
8秒前
魁魁完成签到,获得积分20
8秒前
自由的明雪完成签到,获得积分10
9秒前
粱从寒发布了新的文献求助10
9秒前
10秒前
梅竹完成签到,获得积分10
10秒前
guo_a_n完成签到,获得积分10
10秒前
MMX完成签到,获得积分10
10秒前
nenenn完成签到,获得积分10
10秒前
asss完成签到,获得积分10
11秒前
小杨完成签到,获得积分10
11秒前
Eton完成签到,获得积分10
11秒前
11秒前
量子星尘发布了新的文献求助10
11秒前
Ava应助wmw采纳,获得10
12秒前
13秒前
13秒前
非鱼鱼子发布了新的文献求助10
14秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968964
求助须知:如何正确求助?哪些是违规求助? 3513877
关于积分的说明 11170569
捐赠科研通 3249201
什么是DOI,文献DOI怎么找? 1794692
邀请新用户注册赠送积分活动 875297
科研通“疑难数据库(出版商)”最低求助积分说明 804755