Hypochlorous Acid-Activatable NIR Fluorescence/Photoacoustic Dual-Modal Probe with High Signal-to-Background Ratios for Imaging of Liver Injury and Plasma Diagnosis of Sepsis

Hypochlorous acid can be employed as a biomarker for blood infection (such as sepsis) and tissue damage (such as drug-induced liver injury, DILI), and the diagnosis of tissue damage or blood infection can be achieved through the detection of hypochlorous acid in relevant biological samples. Considering the complex environment and the diverse interferences in living organisms and blood plasma, developing new detection methods for HClO with high signal-to-background ratios is particularly important, and it can improve the accuracy of detection and quality of imaging based on a higher contrast, which makes the detection of HClO clearer and more accurate. Here, based on the advantages of the NIR fluorescence/photoacoustic dual-modal probe, we reported a hypochlorous acid-activatable NIR fluorescence/photoacoustic dual-modal probe (NIRF-PA-HClO) based on the spirolactam ring-opening strategy in this paper. NIRF-PA-HClO showed excellent NIRF/PA dual-modal responses with high SBRs for HClO in solution, cells, and mice. Moreover, NIRF-PA-HClO was successfully applied for high-contrast imaging of DILI. Finally, NIRF-PA-HClO was employed for the blood plasma diagnosis of sepsis with satisfactory results. In summary, the above results proved that NIRF-PA-HClO would be a potentially useful tool for the study of physiological and pathological roles of HClO, the investigation of the pathology and therapeutic mechanisms of hepatotoxicity, and the diagnosis of blood infection. Also, the development of NIRF-PA-HClO provides new design mentality for constructing other analyte-activatable NIRF/PA dual-modal probes with high SBRs.