脱甲基酶
拟南芥
突变体
生物
表观遗传学
组蛋白甲基化
组蛋白
染色质
细胞生物学
DNA甲基化
组蛋白甲基转移酶
遗传学
生物化学
基因表达
基因
作者
Di Fan,Xinqiang Wang,Tingting Liu,Huimin Liu,Yingying Pan,Xiaofeng Tang,Ye Xiao,Kuan Sun,Yuchen Yue,Dan Xu,Chaofeng Li,Keming Luo
摘要
Summary In plant species, anthocyanin accumulation is specifically regulated by light signaling. Although the CONSTITUTIVELY PHOTOMORPHOGENIC1/SUPPRESSOR OF PHYA‐105 (COP1/SPA) complex is known to control anthocyanin biosynthesis in response to light, the precise mechanism underlying this process remains largely unknown. Here, we report that Increase in BONSAI Methylation 1 (IBM1), a JmjC domain‐containing histone demethylase, participates in the regulation of light‐induced anthocyanin biosynthesis in Arabidopsis . The expression of IBM1 was induced by high light (HL) stress, and loss‐of‐function mutations in IBM1 led to accelerated anthocyanin accumulation under HL conditions. We further identified that IBM1 is directly associated with SPA1/3/4 chromatin in vivo to establish a hypomethylation status on H3K9 and DNA non‐CG at these loci under HL, thereby releasing their expression. Genetic analysis showed that quadruple mutants of IBM1 and SPA1/3/4 resemble spa134 mutants. Overexpression of SPA1 in ibm1 mutants complements the mutant phenotype. Our results elucidate the significance and mechanism of IBM1 histone demethylase in the epigenetic regulation of anthocyanin biosynthesis in Arabidopsis under HL conditions.
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