核糖核酸
内含子
生物
计算生物学
人类免疫缺陷病毒(HIV)
病毒复制
序列(生物学)
基因组
病毒学
细胞生物学
基因
遗传学
病毒
作者
Patrick E. H. Jackson,Godfrey Dzhivhuho,Jing Huang,Marie‐Louise Hammarskjöld,David Rekosh
标识
DOI:10.1007/978-1-0716-3862-0_13
摘要
Retroviruses must overcome cellular restrictions to the nucleocytoplasmic export of viral mRNAs that retain introns in order to complete their replication cycle. HIV accomplishes this using a system comprised of a trans-acting viral protein, Rev, and a cis-acting RNA secondary structure in the viral genome, the Rev-Response Element (RRE). HIV primary isolates differ with respect to the sequence and functional activity of the Rev-RRE system. Here, we describe a high throughput assay system for analyzing Rev-RRE functional activity using packageable viral vectors.
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