亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Interferon-induced polarization of M1 macrophages mediates antiviral activity against the hepatitis B virus via the hepcidin-ferroportin axis

海西定 铁转运蛋白 病毒学 干扰素 病毒 医学 免疫学 炎症
作者
Qian Liu,Jianfei Li,Qiyin Zong,Zhi Duan,Futing Liu,Wanlu Duan,Mengqi Ruan,Hao Zhang,Yan Liu,Qiang Zhou,Qin Wang
出处
期刊:International Immunopharmacology [Elsevier BV]
卷期号:134: 112219-112219 被引量:1
标识
DOI:10.1016/j.intimp.2024.112219
摘要

Given its ability to inhibit HBV replication, Interferon alpha (IFN-α) treatment has been confirmed to be effective in managing Chronic Hepatitis B (CHB). However, its underlying mechanisms are incompletely understood. Herein, we investigated the antiviral properties of IFN-α by introducing IFN-α expression plasmids into a well-established HBV Hydrodynamic Injection (HDI) mouse model and examined the impact of IFN-α or hepcidin treatment on macrophages derived from THP-1 cells. The cytokine profiles were analyzed using the cytometry microsphere microarray technology, and flow cytometry was used to analyze the polarization of macrophages. Additionally, the IL-6/JAK2/STAT3 signaling pathway and the hepcidin-ferroportin axis were analyzed to better understand the macrophage polarization mechanism. As evidenced by the suppression of HBV replication, injection of an IFN-α expression plasmid and supernatants of IFN-α-treated macrophages exerted anti-HBV effects. The IFN-α treatment up-regulated IL-6 in mice with HBV replication, as well as in IFN-α-treated HepG2 cells and macrophages. Furthermore, JAK2/STAT3 signaling and hepcidin expression was promoted, inducing iron accumulation via the hepcidin-ferroportin axis, which caused the polarization of M1 macrophages. Furthermore, under the effect of IFN-α, IL-6 silencing or blockade downregulated the JAK2/STAT3 signaling pathway and hepcidin, implying that increased hepcidin expression under IFN-α treatment was dependent on the IL-6/JAK2/STAT3 pathway. The IL-6/JAK2/STAT3 signaling pathway is activated by IFN-α which induces hepcidin expression. The resulting iron accumulation then induces the polarization of M1 macrophages via the hepcidin-ferroportin axis, yielding an immune response which exerts antiviral effects against HBV replication.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
奥黛丽赫本完成签到,获得积分10
刚刚
鹿茸与共完成签到,获得积分10
刚刚
酷波er应助lv采纳,获得10
1秒前
2秒前
鹿茸与共发布了新的文献求助10
3秒前
enchanted发布了新的文献求助10
4秒前
13秒前
Hqing完成签到 ,获得积分10
15秒前
18秒前
19秒前
NIUB完成签到,获得积分10
22秒前
直率芮完成签到 ,获得积分10
23秒前
domingo发布了新的文献求助10
24秒前
Crisp完成签到 ,获得积分10
26秒前
28秒前
enchanted发布了新的文献求助10
29秒前
32秒前
栗栗栗子发布了新的文献求助10
32秒前
enchanted发布了新的文献求助10
42秒前
栗栗栗子完成签到,获得积分10
43秒前
喝粥不用勺吖完成签到,获得积分20
43秒前
领导范儿应助domingo采纳,获得10
43秒前
个性的秀发完成签到,获得积分10
50秒前
52秒前
耳鼻喉不发言完成签到,获得积分10
54秒前
十六发布了新的文献求助10
56秒前
yj完成签到,获得积分10
59秒前
59秒前
天天完成签到 ,获得积分10
1分钟前
1分钟前
十六完成签到,获得积分10
1分钟前
灵梦柠檬酸完成签到,获得积分10
1分钟前
小m完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
SPLjoker完成签到 ,获得积分10
1分钟前
Wsssss完成签到,获得积分10
1分钟前
奋斗的暖阳完成签到,获得积分10
1分钟前
科研通AI2S应助科研通管家采纳,获得30
2分钟前
CipherSage应助科研通管家采纳,获得10
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990012
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256227
捐赠科研通 3270933
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228