生物
表观遗传学
DNA甲基化
发起人
甲基化
转录因子
遗传学
MYB公司
抄写(语言学)
基因表达调控
基因
基因表达
细胞生物学
语言学
哲学
作者
Ling-Feng Miao,Weiya Xu,Yanhong Liu,Xiangyi Huang,Zhe Chen,Hong Wang,Zihao Wang,Yongming Chen,Qingxin Song,Jing Zhang,Fangpu Han,Huiru Peng,Yingyin Yao,Mingming Xin,Zhaorong Hu,Zhongfu Ni,Qixin Sun,Jiewen Xing,Weilong Guo
摘要
Polyploidization is a major event driving plant evolution and domestication. However, how reshaped epigenetic modifications coordinate gene transcription to generate phenotypic variations during wheat polyploidization is currently elusive. Here, we profiled transcriptomes and DNA methylomes of two diploid wheat accessions (SlSl and AA) and their synthetic allotetraploid wheat line (SlSlAA), which displayed elongated root hair and improved root capability for nitrate uptake and assimilation after tetraploidization. Globally decreased DNA methylation levels with a reduced difference between subgenomes were observed in the roots of SlSlAA. DNA methylation changes in first exon showed strong connections with altered transcription during tetraploidization. Homoeolog-specific transcription was associated with biased DNA methylation as shaped by homoeologous sequence variation. The hypomethylated promoters showed significantly enriched binding sites for MYB, which may affect gene transcription in response to root hair growth. Two master regulators in root hair elongation pathway, AlCPC and TuRSL4, exhibited upregulated transcription levels accompanied by hypomethylation in promoter, which may contribute to the elongated root hair. The upregulated nitrate transporter genes, including NPFs and NRTs, also are significantly associated with hypomethylation, indicating an epigenetic-incorporated regulation manner in improving nitrogen use efficiency. Collectively, these results provided new insights into epigenetic changes in response to crop polyploidization and underscored the importance of epigenetic regulation in improving crop traits.
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