亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

PWWP3A deficiency accelerates testicular senescence in aged mice

精子发生 生物 精母细胞 免疫染色 细胞生物学 下调和上调 染色质免疫沉淀 精子 染色质 男科 减数分裂 基因 基因表达 遗传学 内分泌学 免疫组织化学 免疫学 发起人 医学
作者
Zhen Chen,Cong Liu,Wei Qu,Yan Han,Xiaoyu Zhu,Zejia Li,Dupeng Ma,Mengya Huang,Weihao Gong,Qi Sun,Junhao Lei,Rui Guo,Mengcheng Luo
出处
期刊:International Journal of Andrology [Wiley]
标识
DOI:10.1111/andr.13774
摘要

Abstract Background The PWWP domain‐containing proteins are involved in chromatin‐associated biological processes, including transcriptional regulation and DNA repair, and most of them are significant for gametogenesis and early embryonic development in mammals. PWWP3A, one of the PWWP domain proteins, is a reader of H3K36me2/H3K36me3 and a response factor to DNA damage. However, the physiological role of PWWP3A in spermatogenesis and fertility remains unclear. Objective The goal of this study was to explore the function and mechanism of PWWP3A in the process of spermatogenesis. Materials and methods We generated V5‐Pwwp3a KI mice and PWWP3A polyclonal antibody to observe the localization of PWWP3A in vivo. Meanwhile, Pwwp3a KO mice was used to explore the function in spermatogenesis. Results We reported that PWWP3A is a predominant expression in the testis of mice. During spermatogenesis, PWWP3A exhibits the temporal expression from early‐pachytene to the round spermatids. The results of spermatocyte spreading and immunostaining showed that PWWP3A aggregated on the XY body, which then diffused as the XY chromosome separated at late‐diplotene. Although the depletion of PWWP3A had no obvious reproductive defects in young male mice, there were observed morphological abnormalities in sperm heads. Immunoprecipitation demonstrated the interaction of PWWP3A with DNA repair proteins SMC5/6; however, PWWP3A deficiency did not result in any meiotic defects. Notably, the testes of aged male Pwwp3a KO mice displayed pronounced degeneration, and were characterized by the presence of vacuolated seminiferous tubules. Furthermore, RNA‐seq analysis revealed an upregulation in the expression of genes which may be involving in immunoregulatory and inflammatory response pathways in aged Pwwp3a KO mice with testicular degeneration. Conclusions Our study showed that PWWP3A was highly enriched in the mouse testis, and the Pwwp3a KO mice were fertile. However, the aged Pwwp3a KO male mice displayed testicular atrophy that may be due to changes in the immune micro‐environment or abnormal repair of DNA damage.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
23秒前
大模型应助科研通管家采纳,获得10
2分钟前
3分钟前
3分钟前
4分钟前
dengxu发布了新的文献求助10
4分钟前
dengxu完成签到,获得积分10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
归尘应助科研通管家采纳,获得10
4分钟前
桐桐应助科研通管家采纳,获得10
4分钟前
4分钟前
4分钟前
受伤白猫发布了新的文献求助10
4分钟前
受伤白猫完成签到 ,获得积分10
5分钟前
5分钟前
6分钟前
summer完成签到,获得积分20
6分钟前
6分钟前
summer发布了新的文献求助10
6分钟前
归尘应助科研通管家采纳,获得10
6分钟前
归尘应助科研通管家采纳,获得10
6分钟前
6分钟前
科研通AI2S应助宣若剑采纳,获得10
6分钟前
7分钟前
7分钟前
7分钟前
8分钟前
ZhuYJ完成签到,获得积分10
8分钟前
ZhuYJ发布了新的文献求助10
8分钟前
香蕉觅云应助ZhuYJ采纳,获得10
8分钟前
lzxbarry完成签到,获得积分0
8分钟前
8分钟前
zyb完成签到 ,获得积分10
8分钟前
归尘应助科研通管家采纳,获得10
8分钟前
归尘应助科研通管家采纳,获得10
8分钟前
Tumumu完成签到,获得积分10
9分钟前
分你一半完成签到 ,获得积分10
10分钟前
10分钟前
Yummy完成签到,获得积分10
10分钟前
Yummy发布了新的文献求助10
10分钟前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Cognitive Paradigms in Knowledge Organisation 2000
Effect of reactor temperature on FCC yield 2000
Near Infrared Spectra of Origin-defined and Real-world Textiles (NIR-SORT): A spectroscopic and materials characterization dataset for known provenance and post-consumer fabrics 610
Promoting women's entrepreneurship in developing countries: the case of the world's largest women-owned community-based enterprise 500
Shining Light on the Dark Side of Personality 400
Introduction to Spectroscopic Ellipsometry of Thin Film Materials Instrumentation, Data Analysis, and Applications 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3307439
求助须知:如何正确求助?哪些是违规求助? 2941053
关于积分的说明 8500304
捐赠科研通 2615430
什么是DOI,文献DOI怎么找? 1428901
科研通“疑难数据库(出版商)”最低求助积分说明 663595
邀请新用户注册赠送积分活动 648461