柠檬黄单胞菌
柑橘溃疡病
效应器
生物
基因沉默
基因表达
黄单胞菌
基因
转录因子
发起人
抄写(语言学)
激活剂(遗传学)
细胞生物学
基因表达调控
溃疡
遗传学
分子生物学
植物
细菌
语言学
哲学
作者
Xinyu Chen,Huasong Zou,Tao Zhuo,Wei Rou,Wei‐Min Wu,Xiaojing Fan
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2024-08-13
卷期号:13
标识
DOI:10.7554/elife.91684.3
摘要
Xanthomonas citri subsp. citri ( Xcc ), the causal agent of citrus canker, elicits canker symptoms in citrus plants because of the transcriptional activator-like (TAL) effector PthA4, which activates the expression of the citrus susceptibility gene CsLOB1 . This study reports the regulation of the putative carbohydrate-binding protein gene Cs9g12620 by PthA4-mediated induction of CsLOB1 during Xcc infection. We found that the transcription of Cs9g12620 was induced by infection with Xcc in a PthA4-dependent manner. Even though it specifically bound to a putative TAL effector-binding element in the Cs9g12620 promoter, PthA4 exerted a suppressive effect on the promoter activity. In contrast, CsLOB1 bound to the Cs9g12620 promoter to activate its expression. The silencing of CsLOB1 significantly reduced the level of expression of Cs9g12620 , which demonstrated that Cs9g12620 was directly regulated by CsLOB1. Intriguingly, PhtA4 interacted with CsLOB1 and exerted feedback control that suppressed the induction of expression of Cs9g12620 by CsLOB1. Transient overexpression and gene silencing revealed that Cs9g12620 was required for the optimal development of canker symptoms. These results support the hypothesis that the expression of Cs9g12620 is dynamically directed by PthA4 for canker formation through the PthA4-mediated induction of CsLOB1.
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