Utilizing a High-throughput Visualization Screening Technology to Develop a Genetically Encoded Biosensor for Monitoring 5-Aminolevulinic Acid Production in Engineered Escherichia coli

5-Aminolevulinic acid (5-ALA) is a non-protein amino acid widely used in agriculture, animal husbandry and medicine. Currently, microbial cell factories are a promising production pathway, but the lack of high-throughput fermentation strain screening tools often hinders the exploration of engineering strategies to increase cell factory yields. Here, mutant AC