谷胱甘肽
化学
活性氧
荧光
荧光寿命成像显微镜
过氧化氢
生物物理学
光化学
纳米颗粒
肿瘤微环境
细胞内
纳米医学
光动力疗法
纳米技术
材料科学
生物化学
癌症研究
肿瘤细胞
有机化学
酶
物理
生物
量子力学
作者
Jun Li,Zu-E Hu,Yun-Jie We,Yanhong Liu,Na Wang,Xiao‐Qi Yu
标识
DOI:10.1016/j.jcis.2021.08.114
摘要
To minimize unwanted reactions with high concentrations of reduced glutathione (GSH) in the tumor microenvironment (TME) during chemodynamic therapy (CDT), a simple and effective strategy was developed to fabricate a TME stimuli-responsive theranostic nanomedicine (Fe-CD) for fluorescence imaging-guided GSH depletion and cancer therapy by combining fluorescent imaging carbon dots (CD) and Fe(III). Introducing Fe(III) into Fe-CD not only quenched the fluorescence of CD while reacting with and consuming intracellular GSH for fluorescence imaging of the depletion of GSH but also provided a source of metal ions to generate more abundant hydroxyl radicals (•OH) with hydrogen peroxide (H2O2) through the Fenton reaction to improve CDT. Fe-CD showed promising •OH generation under H2O2 to effectively degrade methylene blue in vitro and obviously activate the green fluorescence of the reactive oxygen species (ROS) probe in cells. Benefiting from the fluorescence enhancement in response to TME stimulation, Fe-CD greatly enhanced CDT cytotoxicity while monitoring successful GSH depletion by fluorescence imaging. Fe-CD has the potential to act as a theranostic nanomedicine for fluorescence imaging-guided GSH depletion to amplify CDT.
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