扇贝
虾夷盘扇贝
多糖
化学
代谢物
肠道菌群
生物化学
食品科学
新陈代谢
脂质代谢
葡萄糖醛酸
高效液相色谱法
生物
色谱法
生态学
作者
Zhengqi Liu,Yujiao Zhang,Chunqing Ai,Weigong Tian,Chengrong Wen,Shuang Song,Beiwei Zhu
标识
DOI:10.1016/j.foodres.2022.110980
摘要
The prevention of obesity is an urgent need for worldwide public health. The scallop (Patinopecten yessoensis) skirt is the by-product of adductor processing. In order to explore the value of the scallop skirt, the present study isolated an acidic polysaccharide from the skirt of P. yessoensis (SPYP) and evaluated its anti-obesity effect. SPYP was characterized as an acidic heteropolysaccharide with a molecular weight of 13.58 kDa. Through stepwise acid hydrolysis followed by HPLC-MSn analysis, two core chains in SPYP was determined as heparin-like → 4)β-GlcA(1 → 4)α-GlcNAc → and chondroitin sulfate-like → 4)β-GlcA(1 → 3)β-GalNAc →. SPYP could effectively reduce body weight, decrease fat accumulation and prevent high blood lipids induced by a high-fat diet (HFD) in mice. Further 16S ribosomal ribonucleic acid (rRNA) sequencing analysis revealed that the anti-obesity effect of SPYP was notably associated with its modulation on gut microbiota. The short-chain fatty acids were also restored by SPYP supplementation compared to the HFD group. Moreover, liquid chromatography-high resolution accurate mass spectrometry (LC-HRMS) analysis demonstrated that metabolite profiles of HFD-fed mice were altered by SPYP supplementation, especially the lipid metabolism in serum and amino acid metabolism in cecal content, which may contribute to the anti-obesity effect of SPYP. The present study offers the basis for processing and application of P. yessoensis skirt.
科研通智能强力驱动
Strongly Powered by AbleSci AI