[Advances in high-content screening applications in toxicology research].

The toxicity data of chemicals and drugs increases rapidly, while the animal experimental-based tests method could not meet the current demand of health risk assessment. The high-throughput screening techniques based on in vitro alternative models, integrating with computational methods and information technology to establish toxicity tests strategy promises to address this problem. High-content screening (HCS) technology uses automated microscopy and quantitative image platforms to perform multi-parameter and high-throughput phenotypic analysis via a visualization and quantification manner, and to quickly and effectively assess toxicity and prioritization of chemicals, which promotes the development of in vitro toxicity tests and computational toxicology. HCS technology has been included as an important tool for Toxicity Testing in the 21st Century (Tox21) and chemical risk prioritization. Its applications have been widely utilized in the research field of toxicity tests and chemical toxicity mechanisms. In this review, we describe the development of HCS technology, technical points, toxicological applications, and the future directions and challenges of HCS, so as to provide references for the toxicity testing technology and risk assessment methodology.随着各种新、老化学物质健康风险评价的需求急剧增长，基于动物实验的测试方法难以满足当前健康风险评价的需求，迫切需要研发新型的、高通量、灵敏的毒性测试方法，整合基于体外替代模型的高通量筛选技术、计算方法和信息技术的毒性测试策略。其中，高内涵筛选（HCS）利用自动化显微镜和图像分析平台，以可视化和定量的方式对体外模型进行多参数、高通量的表型分析，快速有效评估化学物毒性并分级，推动了体外毒性试验和计算毒理学的发展。HCS技术已被纳入为21世纪毒性测试（Tox21）及化学物风险分级的重要工具，广泛应用于毒性测试和化学物毒作用机制研究。本文从HCS技术的发展、技术要点、毒理学应用以及未来发展的方向及挑战等方面进行综述，以期为毒性测试技术和风险评估方法学提供参考。.