葡萄糖氧化酶
无定形固体
介孔材料
催化作用
级联反应
纳米技术
化学
纳米颗粒
化学工程
级联
材料科学
有机化学
生物传感器
色谱法
工程类
作者
Yuan Ji,Wanning Gao,Shilin Zhang,Bingzhi Li,He Huang,Xing Zhang
出处
期刊:Langmuir
[American Chemical Society]
日期:2022-01-12
卷期号:38 (3): 927-936
被引量:20
标识
DOI:10.1021/acs.langmuir.1c02093
摘要
Integrating nanozymes with natural enzymes to form cascade reactions is one of the most promising ways to develop biocatalysts with versatile performance; however, the applicability of the cascade is typically hampered by the instability of enzymes and the hindrance of mass transfer in the host environment. Utilizing amorphous ZIF-90 (aZIF-90) as a host material, herein, we have reported a one-pot way to encapsulate glucose oxidase (GOx) and magnetic nanoparticles (MNP) to form GOx/MNP@aZIF-90. We reasoned that the amorphous structure of ZIF-90 not only provides a protected environment to confine the cascade reaction but also generates mesopores and internal voids to improve the performance of the enzymatic cascade. The catalytic activity of aZIF-90 was almost 4 times higher than that of crystalline composites, and the residual activity was higher than 80% after being stored for 9 days. This is the first time that GOx and MNP were simultaneously confined in aZIF-90 with mesopores, which suggested that an amorphous metal-organic framework is promising in the development of an enzymatic cascade.
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