溶血磷脂酰胆碱
分析物
药代动力学
色谱法
化学
基质(化学分析)
磷脂酰胆碱
药理学
磷脂
医学
生物化学
膜
作者
Fulin Jiang,Juntao Zheng,Yong Rao,Chang Zhang,Zhi‐Shu Huang,Min Huang,Guoping Zhong
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2022-04-01
卷期号:14 (8): 455-465
被引量:1
标识
DOI:10.4155/bio-2022-0040
摘要
Aim: IQZ23, a novel β-indoloquinazoline derivative, is a potential therapeutic agent for obesity and related metabolic disorders. To assist pharmacokinetics evaluation, a quantitative method for IQZ23 in rat plasma is required. Methods & Results: An LC-MS/MS assay for the determination of IQZ23 in rat plasma was developed and validated for the first time. Chromatographic conditions were optimized to ameliorate matrix effect with direct monitoring of typical phospholipids, including phosphatidylcholine and lysophosphatidylcholine. The structural analog internal standard (SYSU-3d) was set at a proper concentration to avoid analyte sensitivity loss caused by internal standard interference. The well-validated method was employed in the pharmacokinetics study of IQZ23 in Sprague-Dawley rats. Conclusion: This study provided valuable references for the further preclinical study of IQZ23.
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