Immunochemical studies of phospholipids

An effective method for obtaining antibodies against cardiolipin was developed by injection of a complex of cardiolipin antigen (cardiolipin, lecithin and cholesterol) and methylated bovine serum albumin resulting in the formation of high titer anticardiolipin antibodies. Of the lipid components of the antigen, lecithin seems to be important as an auxiliary lipid for the immunogenicity of cardiolipin, while cholesterol is not essential.