癌变
肺癌
基因
表型
癌症研究
基因组DNA
聚合酶链反应
基因组不稳定性
癌症
生物
遗传学
医学
DNA
内科学
DNA损伤
作者
Jasna Banković,Jelena Stojšić,Dragana J. Jovanović,Tijana Stanković,Vedrana Milinković,Sabera Ruždijić,Nikola Tanić
出处
期刊:Lung Cancer
[Elsevier]
日期:2010-02-01
卷期号:67 (2): 151-159
被引量:90
标识
DOI:10.1016/j.lungcan.2009.04.010
摘要
Lung cancer is the most common cause of neoplasia-related death worldwide. One of the crucial early events in carcinogenesis is the induction of genomic instability and mutator phenotype. We investigated genomic instability in 30 patients with non-small-cell lung cancer (NSCLC) by comparing DNA fingerprints of paired tumor and normal tissues using arbitrarily primed polymerase chain reaction (AP-PCR). Selected 21 DNA bands with altered mobility were isolated from polyacrylamide gels, cloned and sequenced. Obtained sequences were submitted to homology search in GenBank database which revealed the following genes: TSPAN14, CDH12, RDH10, CYP4Z1, KIR, E2F4, PHACTR3, PHF20, PRAME family member and SLC2A13. Following the identification of these genes we examined their relation to the clinicopathological parameters and survival of the patients. Our study revealed that genetic alterations of TSPAN14, SLC2A13 and PHF20 appeared prevalently in tumors of grade 1, stage I suggesting that structural changes of these genes could play a role in NSCLC promotion. Contrary to this CYP4Z1, KIR and RDH10 were prevalently mutated in tumors of grade 3, stage III suggesting that they could play a role in NSCLC progression. E2F4, PHACTR3, PRAME family member and CDH12 most probably play important role in NSCLC geneses. In conclusion, our study revealed altered genes previously not described in regard to this type of cancer.
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