天麻
化学
葡聚糖
多糖
高碘酸盐
IC50型
酶
氯仿
肉桂醇
色谱法
残留物(化学)
生物化学
体外
替代医学
中医药
催化作用
病理
选择性
医学
作者
Zhen‐Yuan Zhu,Chun‐Juan Chen,Hui-Qing Sun,Lijing Chen
标识
DOI:10.1080/14786419.2018.1434643
摘要
The structural properties and Angiotensin-I converting enzyme (ACE) inhibition activities of a polysaccharide (PGE) extracted from Gastrodia elata Blume were investigated. PGE was extracted using hot water and purified by Sephadex G-200 followed by ultra-filtration. The structural characterisation of PGE was analysed by FT–IR, NMR spectroscopy, specific rotation determination, periodate oxidation-smith degradation, methylation analysis, GC–MS and Congo red test. The results revealed that PGE was composed by glucose, with an average molecular weight of 1.54 × 103 kDa. The structure of PGE was 1→3 and 1→4,6-branched-glucopyranose that had a linear backbone of (1 → 4)-linked-d-glucopyranose (Glcp). ACE-inhibitory activity results showed that PGE was efficient to inhibit ACE and the IC50 value was 0.66 mg/mL.
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