荧光
斯托克斯位移
检出限
光化学
化学
色谱法
光学
物理
作者
Na Young Kang,Shizeng Pei,Caihong Zhang,Guomei Zhang,Ying Zhou,Li Fan,Qingjia Yao,Wen Wang,Shaomin Shuang,Chuan Dong
标识
DOI:10.1016/j.saa.2020.119409
摘要
A red emitting fluorescence probe, TPA-CPO, based on twisted intra-molecular charge transfer (TICT) was designed and synthesized. The spectra results displayed that TPA-CPO could sense HSA with excellent properties including significant fluorescence enhancement, long emission wavelength, large stokes shift, and wide linear range. The recognition mechanism was proved that TPA-CPO could bind to domain IB of HSA and its TICT process was suppressed by utilizing hydrophobic cavity and low polarity of HSA. TPA-CPO bind to domain IB instead of common drug sites of HSA could effectively avoid interference from most drugs. The selective response of TPA-CPO allowed quantitative detection of HSA with sensitivity limit of 13.65 µg/mL. What’s more, it successfully achieved HSA imaging in HeLa cells.
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