纳米技术
纳米尺度
原子力显微镜
力谱学
化学
显微镜
纳米
生物材料
生物系统
材料科学
物理
生物
光学
复合材料
作者
Daniel J. Müller,Andra C. Dumitru,Cristina Lo Giudice,Hermann E. Gaub,Peter Hinterdorfer,Gerhard Hummer,James J. De Yoreo,Yves F. Dufrêne,David Alsteens
出处
期刊:Chemical Reviews
[American Chemical Society]
日期:2020-11-09
卷期号:121 (19): 11701-11725
被引量:145
标识
DOI:10.1021/acs.chemrev.0c00617
摘要
During the last three decades, a series of key technological improvements turned atomic force microscopy (AFM) into a nanoscopic laboratory to directly observe and chemically characterize molecular and cell biological systems under physiological conditions. Here, we review key technological improvements that have established AFM as an analytical tool to observe and quantify native biological systems from the micro- to the nanoscale. Native biological systems include living tissues, cells, and cellular components such as single or complexed proteins, nucleic acids, lipids, or sugars. We showcase the procedures to customize nanoscopic chemical laboratories by functionalizing AFM tips and outline the advantages and limitations in applying different AFM modes to chemically image, sense, and manipulate biosystems at (sub)nanometer spatial and millisecond temporal resolution. We further discuss theoretical approaches to extract the kinetic and thermodynamic parameters of specific biomolecular interactions detected by AFM for single bonds and extend the discussion to multiple bonds. Finally, we highlight the potential of combining AFM with optical microscopy and spectroscopy to address the full complexity of biological systems and to tackle fundamental challenges in life sciences.
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