Soluble Pearl Extract provides effective skin lightening by antagonizing endothelin

Abstract Background Incidence of skin pigmentation disorders has been on the rise globally. This calls for safer and more effective topical skin lightening and freckle‐removing products. In this study, we hypothesized that Soluble Pearl Extract (SPE) may possess endothelin antagonizing compounds with good skin whitening effects. Objectives (a) To determine the effect and mechanisms of SPE on ET‐1‐treated B16 melanoma cells. (b) To explore the cytotoxic effects of SPE on B16 melanoma cells. Methods CCK‐8 assay was performed to determine how SPE and ET‐1 affect the proliferation rate of B16 melanoma cells, the NaOH lysis assay was conducted to quantify the content of melanin while the tyrosinase activity was determined by DOPA oxidation test. The mRNA and protein expression levels of TYR and TRP‐1 were determined by qRT‐PCR assay and Western blot assay, respectively. Results We found that SPE at 0.1 and 1 μg/mL concentrations has no effect on the proliferation of the cells and 10 nmol/L ET‐1 promoted B16 melanoma cells proliferation. Notably, B16 melanoma cells treated with 10 nmol/L ET‐1 exhibited significantly higher melanin synthesis, tyrosinase activity, TYR, and TRP‐1 mRNA expression levels compared with untreated cells. Of note, the effects of 10 nmol/L ET‐1 treatment were abolished with SPE in a dose‐dependent manner. Conclusions SPE inhibits endothelin thereby safely and effectively lightening lightens the skin by antagonizing endothelin. Moreover, SPE is safe and effective.