Characterizing metabolites and potential metabolic pathways changes to understanding the mechanism of medicinal plant Phellodendri Amurensis cortex against doxorubicin-induced nephritis rats using UPLC-Q/TOF-MS metabolomics

化学 代谢组学 代谢物 尿 药理学 肾皮质 色谱法 内科学 生物化学 医学
作者
Hongcai Zhang,Shuxiang Zhang,Wenran Wang,Qi Wang,Haixue Kuang,Qiuhong Wang
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier BV]
卷期号:188: 113336-113336 被引量:14
标识
DOI:10.1016/j.jpba.2020.113336
摘要

Phellodendri Amurensis cortex (PAC), a famous traditional Chinese herb with good anti-inflammatory efficacy, is used to treat various liver and kidney sickness in clinical practice. However, the potential mechanisms protecting against nephritis of PAC have not been comprehensively elucidated. The aim of this research was to explore the mechanism of PAC against doxorubicin- induced nephritis in rats by characterizing metabolites and potential metabolic pathways changes. The rat models of nephritis were established using 6.5 mg/kg doxorubicin injection from caudal vein for five weeks. The rats in the treatment group were respectively received PAC extract at the dose of 216, 432, and 864 mg/kg once a day during the experiment. Then, urine metabolomics strategy based on ultra-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry (UPLC-Q/TOF-MS) has been employed to discover the possible significant metabolites and metabolic pathway of nephritis rats. At the end of the experiment, serum, urine and kidney tissue were collected for biochemical and pathological examination. The results showed that PAC treatment notably decreased urinary protein, serum Cr content and renal tissue lesions, and increased serum TP and ALB content. A total of potential twenty- eight metabolites such as 5'-methylthioadenosine, cGMP, dehydroepiandrosterone sulfate, salbuta, 2-phenylaminoadenosine contributing to nephritis rat model were selected and identified in the urine samples. Compared with the model group, the high-dose PAC group can recall 18 metabolites level, the medium-dose group can recall 13 metabolites level, and the low-dose PAC group can recall 8 metabolites level, which were involved in nine primary metabolic pathways such as steroid hormone biosynthesis, alanine,aspartate and glutamate metabolism, cysteine and methionine metabolism as well as glyoxylate and dicarboxylate metabolism. The protein expressions of key enzymes involving methylthioadenosine phosphorylase (Mtap), cytidine deaminase (Cda), thymidine kinase (Tk), argininosuccinate synthase (Ass) in metabolic pathways were further verified by Western blot. The results showed that Phellodendron chinense up-regulated the protein expressions of Cda and Tk and down-regulated the protein expressions of Mtap and Ass. In conclusion, PAC possesses renoprotective effect against doxorubicin-induced nephritis, which may be mediated via regulating differential metabolites, reducing oxidative stress response, improving renal function, enhancing the ability of the immune system, regulating the role of key enzymes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
xiangwang完成签到 ,获得积分10
6秒前
Joan_89完成签到,获得积分10
9秒前
juliar完成签到 ,获得积分10
17秒前
Akim应助DeepLearning采纳,获得10
21秒前
迈克老狼完成签到 ,获得积分10
22秒前
Kelevator完成签到,获得积分10
27秒前
DeepLearning完成签到,获得积分20
28秒前
31秒前
DeepLearning发布了新的文献求助10
34秒前
SOL完成签到 ,获得积分10
49秒前
丝丢皮的完成签到 ,获得积分10
52秒前
t铁核桃1985完成签到 ,获得积分10
52秒前
丝丢皮得完成签到 ,获得积分10
58秒前
爆米花应助DeepLearning采纳,获得10
1分钟前
铜豌豆完成签到 ,获得积分10
1分钟前
cheng完成签到,获得积分10
1分钟前
君看一叶舟完成签到 ,获得积分10
1分钟前
在水一方应助科研通管家采纳,获得10
1分钟前
1分钟前
Chuang完成签到 ,获得积分10
1分钟前
热心市民完成签到 ,获得积分10
1分钟前
吃不胖的魔芋丝完成签到 ,获得积分10
1分钟前
1分钟前
李东东完成签到 ,获得积分10
1分钟前
fsznc1完成签到 ,获得积分0
1分钟前
年轻的笙完成签到,获得积分10
1分钟前
嘻嘻完成签到 ,获得积分10
1分钟前
huiluowork完成签到 ,获得积分10
2分钟前
2分钟前
拾陆发布了新的文献求助10
2分钟前
乔杰完成签到 ,获得积分10
2分钟前
ramsey33完成签到 ,获得积分10
2分钟前
小静完成签到 ,获得积分10
2分钟前
默笙完成签到 ,获得积分10
2分钟前
小乙猪完成签到 ,获得积分0
2分钟前
赵帅完成签到 ,获得积分10
2分钟前
2分钟前
西扬完成签到 ,获得积分10
2分钟前
2分钟前
Ray完成签到 ,获得积分10
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990835
求助须知:如何正确求助?哪些是违规求助? 3532241
关于积分的说明 11256614
捐赠科研通 3271100
什么是DOI,文献DOI怎么找? 1805229
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809236