芦丁
金丝桃苷
异鼠李素
山奈酚
色谱法
黄酮醇
化学
槲皮素
磷酸
传统医学
生物化学
医学
有机化学
抗氧化剂
作者
Homa Hajimehdipoor,Babak Mokhtari kondori,Gholam R. Amin,Noushin Adib,Hossein Rastegar,Maryam Shekarchi
出处
期刊:DARU
[Springer Nature]
日期:2012-10-16
卷期号:20 (1)
被引量:49
标识
DOI:10.1186/2008-2231-20-57
摘要
Abstract Background Cuscuta species known as dodder, have been used in traditional medicine of eastern and southern Asian countries as liver and kidney tonic. Flavonoids are considered as the main biologically active constituents in Cuscuta plants especially in C. chinensis Lam. Objective In the present study, a fast, simple and reliable method for the simultaneous determination and quantization of C. chinensis flavonols including hyperoside, rutin, isorhamnetin and kaempferol has been developed. Materials and methods The chromatographic separation was carried out on a reversed phase ACE 5 C 18 with eluting at a flow rate of 1 ml/min using a gradient with O -phosphoric acid 0.25% : acetonitrile for 42 min. UV spectra were collected across the range of 200–900 nm, extracting 360 nm for the chromatograms. The method was validated according to linearity, selectivity, precision, recovery, LOD and LOQ. Results The method was selective for determination of rutin, hyperoside, isorhamnetin and kampferol. The calibration graphs of flavonols were linear with r 2 > 0.999. RSDs% of intra- and inter-day precisions were found 1.3&3.4 for rutin, 1.5&2.8 for hyperoside, 1.3&3.3 for isorhamnetin and 1.7 & 2.9 for kaempferol which were satisfactory. LODs and LOQs were calculated as 1.73 & 8.19 for rutin, 0.09 & 4.19 for hyperoside, 2.09 & 6.3 for isorhamnetin and 0.18 & 0.56 for kaempferol. The recovery averages of above-mentioned flavonols were 90.3%, 97.4%, 98.7% and 90.0%, respectively. Conclusion The simplicity of the method makes it highly valuable for quality control of C. chinensis according to quantization of flavonols.
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