扫描电镜
显微镜
荧光显微镜
树突棘
超分辨率
生物
皮质(解剖学)
神经科学
大脑皮层
分辨率(逻辑)
生物物理学
荧光
光学
受激发射
物理
海马结构
人工智能
图像(数学)
计算机科学
激光器
作者
Sebastian Berning,Katrin I. Willig,Heinz Steffens,Payam Dibaj,Stefan W. Hell
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2012-02-03
卷期号:335 (6068): 551-551
被引量:333
标识
DOI:10.1126/science.1215369
摘要
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.
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