生物
发起人
操纵子
抄写(语言学)
响应元素
反终止
转录因子
非翻译区
电箱
分子生物学
三素数非翻译区
基因
一般转录因子
基因表达
大肠杆菌
遗传学
信使核糖核酸
哲学
语言学
作者
Peng Xue,David F. Corbett,Marie Goldrick,Clare Naylor,Ian S. Roberts
摘要
ABSTRACT Escherichia coli group 2 capsule gene clusters are temperature regulated, being expressed at 37°C but not at 20°C. Expression is regulated at the level of transcription by two convergent promoters, PR1 and PR3. In this paper, we show that regulation of transcription from PR3 involves a number of novel features including H-NS, SlyA, and a large 741-bp 5′ untranslated region (UTR). H-NS represses transcription from PR3 at 20°C and binds both 5′ and 3′ of the transcription start site. The 3′ downstream regulatory element (DRE) was essential for temperature-dependent H-NS repression. At 37°C, SlyA activates transcription independent of H-NS but maximal transcription requires H-NS. The UTR is present between the transcription start site and the first gene in the operon, kpsM . We demonstrate that the UTR, as well as containing the H-NS DRE, functions to moderate the extent of transcription that reaches kpsM and allows the binding of antitermination factor RfaH.
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