亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Fatty-acid oxidation and calcium homeostasis are involved in the rescue of bupivacaine-induced cardiotoxicity by lipid emulsion in rats*

布比卡因 心脏停搏 心脏毒性 医学 射血分数 脂肪乳剂 麻醉 线粒体通透性转换孔 丸(消化) 心功能曲线 脂肪酸 药理学 毒性 内科学 肠外营养 化学 生物化学 心力衰竭 细胞凋亡 程序性细胞死亡
作者
Parisa Partownavid,Soban Umar,Jingyuan Li,Siamak Rahman,Mansoureh Eghbali
出处
期刊:Critical Care Medicine [Ovid Technologies (Wolters Kluwer)]
卷期号:40 (8): 2431-2437 被引量:107
标识
DOI:10.1097/ccm.0b013e3182544f48
摘要

Objectives: Lipid emulsion has been shown to be effective in resuscitating bupivacaine-induced cardiac arrest but its mechanism of action is not clear. Here we investigated whether fatty-acid oxidation is required for rescue of bupivacaine-induced cardiotoxicity by lipid emulsion in rats. We also compared the mitochondrial function and calcium threshold for triggering of mitochondrial permeability transition pore opening in bupivacaine-induced cardiac arrest before and after resuscitation with lipid emulsion. Design: Prospective, randomized animal study. Setting: University research laboratory. Subjects: Adult male Sprague-Dawley rats. Interventions: Asystole was achieved with a single dose of bupivacaine (10 mg/kg over 20 secs, intravenously) and 20% lipid emulsion infusion (5 mL/kg bolus, and 0.5 mL/kg/min maintenance), and cardiac massage started immediately. The rats in CVT-4325 (CVT) group were pretreated with a single dose of fatty-acid oxidation inhibitor CVT (0.5, 0.25, 0.125, or 0.0625 mg/kg bolus intravenously) 5mins prior to inducing asystole by bupivacaine overdose. Heart rate, ejection fraction, fractional shortening, the threshold for opening of mitochondrial permeability transition pore, oxygen consumption, and membrane potential were measured. The values are mean ± SEM. Measurements and Main Results: Administration of bupivacaine resulted in asystole. Lipid Emulsion infusion improved the cardiac function gradually as the ejection fraction was fully recovered within 5 mins (ejection fraction = 64 ± 4% and fractional shortening = 36 ± 3%, n = 6) and heart rate increased to 239 ± 9 beats/min (71% recovery, n = 6) within 10 mins. Lipid emulsion was only able to rescue rats pretreated with low dose of CVT (0.0625 mg/kg; heart rate ~ 181 ± 11 beats/min at 10 mins, recovery of 56%; ejection fraction = 50 ± 1%; fractional shortening = 26 ± 0.6% at 5 mins, n = 3), but was unable to resuscitate rats pretreated with higher doses of CVT (0.5, 0.25, or 0.125 mg/kg). The calcium-retention capacity in response to Ca2+ overload was significantly higher in cardiac mitochondria isolated from rats resuscitated with 20% lipid emulsion compared to the group that did not receive Lipid Emulsion after bupivacaine overdose (330 ± 42 nmol/mg vs. 180 ± 8.2 nmol/mg of mitochondrial protein, p < .05, n = 3 in each group). The mitochondrial oxidative rate and membrane potential were similar in the bupivacaine group before and after resuscitation with lipid emulsion infusion. Conclusions: Fatty-acid oxidation is required for successful rescue of bupivacaine-induced cardiotoxicity by lipid emulsion. This rescue action is associated with inhibition of mitochondrial permeability transition pore opening.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
24秒前
核桃发布了新的文献求助10
29秒前
田様应助Monet采纳,获得10
29秒前
40秒前
凶狠的寄风完成签到 ,获得积分10
43秒前
Monet发布了新的文献求助10
45秒前
52秒前
A水暖五金批发张哥完成签到,获得积分10
54秒前
tan发布了新的文献求助20
1分钟前
郗妫完成签到,获得积分10
1分钟前
情怀应助科研通管家采纳,获得10
1分钟前
生动土匪发布了新的文献求助10
1分钟前
缓慢的枫叶应助tan采纳,获得60
1分钟前
1分钟前
凉月发布了新的文献求助10
1分钟前
大风车发布了新的文献求助10
1分钟前
凉月完成签到,获得积分20
2分钟前
科研通AI2S应助凉月采纳,获得10
2分钟前
柴三岁完成签到,获得积分20
3分钟前
酷波er应助科研通管家采纳,获得10
3分钟前
顾君如完成签到 ,获得积分10
3分钟前
汉堡包应助柴三岁采纳,获得10
3分钟前
止戈完成签到 ,获得积分10
3分钟前
4分钟前
zhangxr发布了新的文献求助10
4分钟前
科研通AI2S应助zhangxr采纳,获得10
4分钟前
5分钟前
柴三岁发布了新的文献求助10
5分钟前
xx完成签到 ,获得积分10
5分钟前
溜圈吃不胖完成签到,获得积分10
5分钟前
123发布了新的文献求助10
5分钟前
6分钟前
小叶同学发布了新的文献求助10
6分钟前
lixuebin完成签到 ,获得积分10
7分钟前
小马甲应助小叶同学采纳,获得10
7分钟前
一串数字发布了新的文献求助10
7分钟前
h0jian09完成签到,获得积分10
8分钟前
汉堡包应助XiaoXiao采纳,获得10
8分钟前
kk发布了新的文献求助10
9分钟前
kk完成签到,获得积分10
9分钟前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3162323
求助须知:如何正确求助?哪些是违规求助? 2813330
关于积分的说明 7899688
捐赠科研通 2472818
什么是DOI,文献DOI怎么找? 1316526
科研通“疑难数据库(出版商)”最低求助积分说明 631365
版权声明 602142