脱氧核酶
化学
血红素
检出限
DNA
G-四倍体
分析物
辣根过氧化物酶
分子信标
纳米技术
生物传感器
寡核苷酸
核酸
组合化学
滚动圆复制
生物物理学
酶
生物化学
色谱法
生物
材料科学
血红素
作者
Simcha Shimron,Fuan Wang,Ron Orbach,Itamar Willner
摘要
An enzyme-free amplified detection platform is described using the horseradish peroxidase (HRP)-mimicking DNAzyme as an amplifying label. Two hairpin structures that include three-fourths and one-fourth of the HRP-mimicking DNAzyme in caged, inactive configurations are used as functional elements for the amplified detection of the target DNA. In the presence of the analyte DNA, one of the hairpins is opened, and this triggers the autonomous cross-opening of the two hairpins using the strand displacement principle. This leads to the formation of nanowires consisting of the HRP-mimicking DNAzyme. The resulting DNA nanowires act as catalytic labels for the colorimetric or chemiluminescent readout of the sensing processes (the term “enzyme-free” refers to a protein-free catalyst). The analytical platform allows the sensing of the analyte DNA with a detection limit corresponding to 1 × 10–13 M. The optimized system acts as a versatile sensing platform, and by coaddition of a “helper” hairpin structure any DNA sequence may be analyzed by the system. This is exemplified with the detection of the BRCA1 oncogene with a detection limit of 1 × 10–13 M.
科研通智能强力驱动
Strongly Powered by AbleSci AI