图像分辨率
共焦显微镜
显微镜
物理
光学切片
光谱成像
生物医学工程
光学成像
作者
Arthur Davis,Olivier Levecq,Hicham Azimani,David Siret,Arnaud Dubois
标识
DOI:10.1364/boe.10.000694
摘要
Line-field confocal optical coherence tomography (LC-OCT) operating in two distinct spectral bands centered at 770 nm and 1250 nm is reported, using a single supercontinuum light source and two different line-scan cameras. B-scans are acquired simultaneously in the two bands at 4 frames per second. Greyscale representation and color fusion of the images are performed to either produce a single image with both high resolution (1.3 µm × 1.2 µm, lateral × axial, measured at the surface) in the superficial part of the image and deep penetration, or to highlight the spectroscopic properties of the sample. In vivo images of fair and dark skin are presented with a penetration depth of ∼700 µm.
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