Wheat Cell Number Regulator CNR10 Enhances the Tolerance, Translocation, and Accumulation of Heavy Metals in Plants

Heavy metal contamination affects crop growth and development and can indirectly threaten human health. Therefore, improving the content of microelements and reducing the accumulation of toxic metals by genetic breeding in crops is an effective strategy to solve this environmental problem. Previous reports show plant cadmium resistance (PCR) protein can transport zinc (Zn) and cadmium (Cd). The cell number regulator (CNR) protein, which functions to regulate organ size, has high similarity to, and shares conserved motifs with, PCR. Therefore, CNR may be involved in regulating heavy metal translocation. We isolated TuCNR10 from diploid wheat, Triticum urartu. Real-time quantitative PCR showed TuCNR10 expression increased in the shoots and roots of seedlings under Cd, Zn, and manganese (Mn) stresses. Confocal imaging indicated TuCNR10 was localized at the plasma membrane. Overexpression of TuCNR10 in Arabidopsis and rice enhanced Cd, Zn, and Mn tolerance and improved Cd, Zn, and Mn translocation from roots to shoots. Compared with wild-type rice, rice overexpressing TuCNR10 had lower Cd and higher Zn and Mn contents in grains. These results indicated that TuCNR10 may be a transporter of Cd, Zn, and Mn. TuCNR10 may be a useful genetic resource for microelement fortification and reducing toxic metal accumulation in crops.