Some probiotic potential of Weissella confusa MD1 and Weissella cibaria MD2 isolated from fermented batter

This study was conducted to evaluate the probiotic potential of Weissella confusa MD1 and Weissella cibaria MD2 isolated from a fermented batter. They possessed characters like high survivability in gastrointestinal conditions, tolerance towards lysozyme and phenol. Both strains exhibited strong auto-aggregation property and co-aggregation with Listeria monocytogenes. The adhesion behavior towards intestinal epithelial cells (HT-29 cells) was observed by scanning electron microscopy (SEM). Both strains MD1 and MD2 exhibited antimicrobial activity against foodborne pathogens like Escherichia coli, Salmonella enterica, Listeria monocytogenes, Salmonella typhi and Staphyloccocus aureus. They also showed high bile salt hydrolase activity towards sodium taurocholate and bile salt mixture. Strain MD1 and MD2 demonstrated a tremendous reduction in the cholesterol by 67.11% and 78%, respectively. The intact cells of both strains showed strong antioxidant activity by scavenging 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical and inhibition of linoleic acid peroxidation. The formation of biofilm was demonstrated by using atomic force microscopy (AFM) on the glass cover. Both strains showed sensitivity towards conventionally used antibiotics and no hemolytic activity. The results from the study suggested that Weissella confusa MD1 and Weissella cibaria MD2 have exemplary technological properties that can be implemented in the pharmaceutical and food industry.