Cartilage oligomeric matrix protein affects the biological behavior of papillary thyroid carcinoma cells by activating the PI3K/AKT/Bcl-2 pathway

软骨寡聚基质蛋白 小发夹RNA PI3K/AKT/mTOR通路 蛋白激酶B 癌症研究 化学 基因沉默 甲状腺癌 流式细胞术 甲状腺癌 细胞生物学 分子生物学 细胞生长 细胞凋亡 生物 癌症 病理 医学 内分泌学 甲状腺 基因 基因敲除 生物化学 遗传学 替代医学 骨关节炎
作者
Jirong Zhang,Hongzhi Wang,Chunpeng Lv,Jun Han,Mingyu Hao,Jingjing Li,Hong Qiao
出处
期刊:Journal of Cancer [Ivyspring International Publisher]
卷期号:12 (6): 1623-1633 被引量:13
标识
DOI:10.7150/jca.49144
摘要

Objective: To explore the effect of cartilage oligomeric matrix protein (COMP) on papillary thyroid carcinoma (PTC).Methods: COMP expression levels in PTC tissues and matched adjacent normal tissues were measured using tissue microarrays.Human PTC cells were cultured and transduced with lentiviral short hairpin RNA against COMP (COMP-shRNA), a negative control (NC) shRNA, or mock transfected (Control).We used the Cell Counting Kit-8, performed colony formation assays, wound healing assays, Transwell invasion assays, flow cytometry, and measured the expression of apoptosis-related proteins at the mRNA and protein levels to explore the effects of COMP on the biological behavior of PTC cells and to discover the specific signaling pathway involved in these processes.Results: COMP expression was significantly higher in PTC tissues than in adjacent normal tissues.At the cellular level, COMP promoted cell migration, increased the invasiveness of PTC cells, and inhibited apoptosis.However, differences in cell proliferation were only observed within 72 hours.At the same time, colony formation assays showed that silencing COMP inhibited the proliferation of PTC cells.We also found that COMP regulated the behavior of PTC cells by activating the PI3K/AKT/Bcl-2 pathway.Conclusions: COMP is upregulated in PTC, which enhances cancer cell invasion and inhibits apoptosis, contributing to the development and progression of PTC.Thus, COMP may serve as a new biomarker for PTC.
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